ASSESSMENT OF ANABAENA WISCONSINENSE (CYANOPROKARYOTA) AS IMMUNOSTIMULANTS OF MALE NILE TILAPIA (OREOCHROMIS NILOTICUS)
Aida
Dawah
Limnology Dept Central Laboratory For Aquaculture Research, Abbassa, Abou- Hammad, Sharkia, Egypt
author
A.
Swilem
Botany Dept., Faculty of Science, Benha University, Egypt
author
Zienab
Nagdy
Limnology Dept Central Laboratory For Aquaculture Research, Abbassa, Abou- Hammad, Sharkia, Egypt
author
Azza
Abd El-Rahman
Fish Diseases Dept. Central Laboratory for Aquaculture Research, Abbassa, Abu-Hammad, Sharkia. Egypt
author
Reham
Abd - El hay
Limnology Dept Central Laboratory For Aquaculture Research, Abbassa, Abou- Hammad, Sharkia, Egypt
author
text
article
2011
eng
A process of assessing the effect of incorporating Cyanoprokaryota Anabaena wisconsinense on immunostimulant and enhancing fish resistance to pathogenic bacteria of Nile tilapia (Oreochromis niloticus) was conducted. One hundred and thirty five of all male O. niloticus (20 ± 2 g / fish) were randomly collected from stored earthen ponds of Abbassa fish farm, Sharkia, Egypt. The fish were acclimatized for two weeks, and then divided into 3 groups (45 fish / group); each in 3 replicates containing (15 fish per / glass aquaria) supplied with 210 liters of water. The first group (T1) was fed with basal diet (crude protein 30%) containing 1% whole fresh cells of A. wisconsinense. The second group (T2) was fed with the same basal diet containing 2% whole cells of A. wisconsinense. The third group (T3) was fed with basal diet free from A. wisconsinense cells (control diet). Fish were hand-fed once daily in 28 days, at a rate of 3% of body weight. At the end of the feeding experiment, 90 fish were challenged (10 fish from each aquarium) with Aeromonas sabriia, Actinobacter anitratus and Aeromonas veronii and then kept under observation for 15 days. A significant increase in Haematocrit level was reported; respiratory burst and serum lysozyme activity among fish fed with diet containing (Cyanoprokaryota) compared to control fish. A. wisconsinense supplement decreased the total bacterial count in the intestine than control group and increased fish resistance to Aeromonas sabriia, Actinobacter anitratus and Aeromonas veronii.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
1
11
https://egyjs.journals.ekb.eg/article_114929_3ac60117e96c5fe3f39c884628870aec.pdf
dx.doi.org/10.21608/egyjs.2011.114929
ISOLATION AND IDENTIFICATION OF SOME INDIGENOUS CYANOPROKARYOTA ISOLATES BASED ON MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERIZATIONS
S.
Salem
Agric. Microbiology Dept., Fac. Agric., Zagazig Univ., Zagazig, Egypt.
author
Fatma
EL-Zamik
Agric. Microbiology Dept., Fac. Agric., Zagazig Univ., Zagazig, Egypt.
author
Howaida
Abd El-Basit
Agric. Microbiology Dept., Fac. Agric., Zagazig Univ., Zagazig, Egypt.
author
Azza
Abd El-All
Soils Water and Environ. Res. Inst., Agric. Res. Center (ARC), Giza, Egypt.
author
A.
Ali
Agric. Microbiology Dept., Fac. Agric., Zagazig Univ., Zagazig, Egypt.
author
text
article
2011
eng
Forty-two cyanoprokaryota strains were isolated from 10 diverse sites along Sharkia Governorate, Egypt (7 sites representing the fertile soils and 3 sites representing the new reclaimed soils). Eight genera and 11 species of N2-fixing cyanoprokaryota were identified based on available morphological distinction and physiological characterization. On the basis of the obtained results, we classified these isolates to 3 genera and 5 species isolated from fertile soils and 7 genera and 8 species isolated from new reclaimed soils. Nostoc spp strains were the most common filamentous heterocyst-forming cyanoprokaryota (22 strains) and two species of Nostoc including 11 strains of N. muscorum and11 strains of N. calicicola. Anabaena includes 10 strains in three species (A. oryzae (6 strains), A. circinalis (2 strains) and A. oscillarioides (2 strains). Nodularia spumigena were represented in this work by two strains from fertile soils. In addition Calothrix clavata (2 strains), Synechococcus sp. (3 strains) and one strain of each Oscillatoria homogenea, Dermocarpa olivacea and Xenococcus kerneri were inhabited in the reclaimed soils. This indicates that Nostoc spp. and Anabaena spp. were highly distributed in fertile soils more than new reclaimed soils. The latter species were the most important cyanoprokaryota genera to fix atmospheric nitrogen in rice field.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
13
32
https://egyjs.journals.ekb.eg/article_114932_41a8e63c0021974da21960a80386f4dd.pdf
dx.doi.org/10.21608/egyjs.2011.114932
BIOCHEMICAL CONSTITUENTS AND BIOACTIVITY OF LAURENCIA OBTUSA (CERAMIALES, RHODOPHYTA) IN RELATION TO SEASONAL AND ECOLOGICAL FACTORS AT RED SEA COAST - EGYPT
Mohamed
Deyab
Botany Department, Faculty of Science at Damietta, Mansoura University, New Damietta, Egypt
author
text
article
2011
eng
Physicochemical analysis of seawater at three sites along the Red Sea cost of Egypt; that is Faied, Ras Gharib and Safaga for one year revealed marked seasonal and local variation in the eutrophication status of the water, temperature and salinity. Laurancia obtusa experienced both local and temporal variation in covering percentage with a maximum dominance during summer and autumn especially at Faied. The contents of proteins, carbohydrates and lipids in L. obtusa tissuesshowed peaks during summer at all sites particularly at Faied. Likewise, the mineral content of the alga exhibited distinct seasonal and local variation. Chloroform extracts of L. obtusa were more efficient as antibacterial than as antifungal. The most sensitive bacterial species was Pseudomonas aeruginosae and the most sensitive fungal species was Fusarium spp. The L. obtusa extract harvested from Safaga was the most potent among the studied sites. The antimicrobial activity of the algal extract obtained from algae collected during autumn and winter was stronger than during summer and spring. This was in marked contrast with the levels of the primary metabolites (protein, lipids and carbohydrates) which showed maximum levels during summer.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
33
46
https://egyjs.journals.ekb.eg/article_114933_24201b6abf293a8b4c511cf5e43971c5.pdf
dx.doi.org/10.21608/egyjs.2011.114933
LABORATORY STUDIES ON THE ALLELOPATHIC ACTIVITY OF THE GREEN ALGA, SCENEDESMUS ACUMINATUS CRUDE EXTRACTS AGAINST THE GREEN ALGA CHLORELLA VULGARIS.
A.
Dawah
Limnology Dept., Central Lab for Aquaculture Research (CLAR), Abbassa Agriculture Research Center, Egypt.
author
M.
Metwaly
Department of Botany, Faculty of Science, Benha University.
author
Z.
Nagdy
Limnology Dept., Central Lab for Aquaculture Research (CLAR), Abbassa Agriculture Research Center, Egypt.
author
I.
Shaker
Limnology Dept., Central Lab for Aquaculture Research (CLAR), Abbassa Agriculture Research Center, Egypt.
author
A.
Mohmed
Limnology Dept., Central Lab for Aquaculture Research (CLAR), Abbassa Agriculture Research Center, Egypt.
author
text
article
2011
eng
The allelopathic activity of the green alga named Scenedesmus acuminatus against the green algaChlorella vulgaris has been reported. Two algae were isolated, purified and identified from water of earthen fish ponds of Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Egypt. S. acuminatus was cultured in mass culture. Algal mass was harvested at exponential growth phase and dried then extracted by the organic solvents (ethanol or methanol) using a soxhlet apparatus. Allelopathic activity of the crude extracts was investigated with agar-well diffusion and paper-disc assay to demonstrate efficiency of antialgal principles against C. vulgaris. Findings revealed that two crude extracts had an inhibitory effect against C. vulgaris where, large and small inhibition zone caused by Agar-well diffusion assay. Ethanolic crude extract was 23.6 and 51.6 mm at concentrations of 100 and 400 µl respectively. The largest and smallest clear zone caused by methanolic crude extract was 36.6 and 20 mm in favor of the above mention concentrations. On the other hand, the largest and smallest clear zone caused by paper- disc assay for ethanolic crude extract was 26.3 and 17.6 mm and for methanolic crude extract was 15.3 and 24 mm at concentrations of 10 and 40 µl respectively.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
47
55
https://egyjs.journals.ekb.eg/article_114936_e8ab1e0e9dea7d073e6a185c61b1257e.pdf
dx.doi.org/10.21608/egyjs.2011.114936
EFFECT OF SEAWEED EXTRACTS AS FOLIAR SPRAY ON SUNFLOWER YIELD AND OIL CONTENT
Hanan
Osman
Botany and Microbiology Depart, Faculty of Science, Al -Azhar University (Girls Branch).
author
Olfat
Salem
Botany and Microbiology Department, Faculty of Science, Helwan University.
author
text
article
2011
eng
A field experiment was conducted, June 2009 to study the effects of foliar applications of different concentrations of seaweed extract of Ulva lactuca (Ulvaceae)and Gracilaria dendroides (Gracilariaceae) on nutrient uptake, growth and yield of sunflower plant. There were five treatment control and two concentration 0.4 and 0.6 % w/v from each algal extract, this treatment applied at 3 times; first one at the seedling stage (20 days after sowing), the second at the flowering stage (40 days after sowing), while the third one before yield stage (70 days after sowing). Application of algal extract significantly increase fresh and dry weight of root and shoot, chlorophyll a, chlorophyll b and carotenoids rather than the control, especially with 0.6% extract of G. dendroides. At harvest stage, application of seaweed significantly increase both root and shoot fresh and dry weight, plant height, head diameter, seed index (weight of 1000 seed), seed yield as compared to control. Also, the highest growth parameters were observed in plant treated by 0.6% extract of G. dendroides, except plant height. Highest oil content was observed at 0.6 % G. dendroides extract, followed with 0.6% U. lactuca extract application (34.05 and 30.55%, respectively). The application of extract of 0.4 % and 0.6 % for both studied algae significantly increased seed yield of sunflower by 20.94, 40.31, 30.29 and 28.73%, respectively, compared to control. Improved nutrient uptake (Na and K) was also observed with seaweed extract applications. Thus, foliar applications of seaweed extracts could be a promising option for yield enhancement and high oil yield production of sunflower plant.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
57
70
https://egyjs.journals.ekb.eg/article_114938_241acfd8abd76d8d633984435b978355.pdf
dx.doi.org/10.21608/egyjs.2011.114938
USE OF SOME EGYPTIAN SEAWEED AS FOLIAR FERTILIZER FOR VICIA FABA L.
Radwa
Khairy
Agriculture Analytical Laboratory Unit, Agric. Fac., Suez Canal Univ.
author
Islam
El-Manawy
Botany Dept., Fac. of Science, Suez Canal Univ.
author
Mohamed
El-Bramawy
Agronomy Dept., Fac. of Agriculture, Suez Canal Univ.
author
text
article
2011
eng
Three common marine algae, Enteromorpha intestinalis, Ulva lactuca and Sarconema filiformis were collected from Suez Canal at Ismailia and chosen to examine their effects as a foliar spray on the growth and yield of faba beans in the Experimental Farm, Fac. of Agric., Suez Canal Univ., Ismailia in November 2006. The aqueous algal extracts were sprayed at concentrations of 0.05, 0.10 and 0.15; each was sprayed four times throughout the vegetative growth period of the plant. The results confirmed that application of eco-friendly fertilizers, derived from marine algae, was effective in increasing the measurements of growth and yield parameters. At the end of the growing season, Vicia faba plants grown with the foliar seaweed application had produced 1.6 to 2.25 folds of the unfertilized controls with using E. intestinalis, 1.7 to 2.4 folds using U. lactuca and 2.5 to 5.6 folds with using S. filiformis. This production may be referred to the minerals, hormone-like substances, amino acids and vitamins in different algae. The present production of broad bean did not reach the Egyptian yield of feddan and this may be attributed to the low PNK in algae. The present study concluded that seaweed products should be boosted with additional supplement of NPK.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
71
88
https://egyjs.journals.ekb.eg/article_114939_64cbc8d2be02b92ed5298862c45b8df3.pdf
dx.doi.org/10.21608/egyjs.2011.114939
NUTRITIONAL COMPOSITION AND MINERAL CONTENT OF FIVE MACROALGAE FROM RED SEA
Nehal
Osman
Botany Department, Faculty of Science, Suez Canal University, Ismailia Governorate, Egypt
author
Islam
El-Manawy
Botany Department, Faculty of Science, Suez Canal University, Ismailia Governorate, Egypt
author
Abeer
Amin
Botany Department, Faculty of Science, Suez Canal University, Ismailia Governorate, Egypt
author
text
article
2011
eng
Nutritional and mineral compositions were evaluated for five seaweed species collected from Red Sea during the winter of 2008. These species were Dictyota divaricata, Padina tetrastromatica, Sargassum subrepandum and Turbinaria triquetra from Phaeophyta and Caulerpa serrulata from Chlorophyta. Moisture contents in algal samples were from 10.6g.100g-1 in Caulerpa serrulata to 23.6g.100g-1 in Turbinaria triquetra. Other species had moisture contents more close to C. serrulata. Total dietary fiber was the most abundant component in these algae (18.6% to 55.41%), followed by ash content (17.057 to 29.573 g.100g-1) and carbohydrates (14.6 to 45.6 100g-1). Proteins in all species were from 3.87% to 14.48%. D. divaricata had the highest content of total lipids (10.51%); meanwhile other species showed (4.24-1.62%). The five algal species contained large amounts of Na, Ca, K and Fe, moderate amounts of Zn, and were lower in Cu, Cd, Ni and Mn. Pb was measured as 0.001 ppm in P. tetrastromatica and was absent in the other species.
Egyptian Journal of Phycology
The Egyptian Phycological Society
1110-8649
12
v.
1
no.
2011
89
102
https://egyjs.journals.ekb.eg/article_114940_7114e2b5a8c06db22845cbf2df6322e7.pdf
dx.doi.org/10.21608/egyjs.2011.114940