THE IDENTIFICATION OF SOME DUNALIELLA SPECIES UTILIZING THE GENETIC RELATEDNESS OF PROTEIN, ISOZYME AND DNA BANDING PATTERNS.

Document Type : Original Article

Authors

1 Genetic Engineering and Biotechnology Institute - Minoufiya University.

2 Botany Department - Faculty of Science- Alexandria

3 Botany Department - Faculty of Science- Alexandria University- Alexandria – Egypt

Abstract

Although the different species of Dunaliella are nearly identified but some species in culture collection are still misnamed. In this work the primary and tertiary semantides were analyzed for the identification of four species of Dunaliella. The results of tertiary semantides including protein profile and isozymes pattern revealed that there is no species relation between the four species specially between D. salina and D. bardawil. The results of PCR-RFLP-DNA banding pattern strengthened those obtained from the isozymes and protein profile pattern and proved that D. bardawil is neither a synonymous species of D. salina nor of D. parva but D. bardawil should be regarded as separate species. This basic molecular biology technique recommended to be used in ascertaining the genetic relatedness and diversity of taxa.

References

Abd El-Salam, A.Z.; El-Adl, A.M.; Kosba, Z.A. and Adham, I.M. (1981). Isozyme polymorphism in Drosophila. Egypt. J. Genet. Cytol. 10:210- 215.

Abd-El-kareem,M.S.M. (1993). Some chemotaxonomic studies on members of Rhodophyceae growing along the mediterranean sea- shore of Alexandria. Ph.D. Thesis. Fac of Sci. Alex. Univ. Alex. Egypt.
Abd-El-Salam, E. M.F. (1997). Comparative taxonomic studies on Charophytes in relation to other green plants.Ph. D. Thesis, Fac.of Sci. Alex. Univ. Alexandria, Egypt.
Ashraf, B. A.H. (1998). Genetic studies on some microalgae. M.Sc. Thesis, Fac.of Agriculture,  Ain Shams Univ. Cairo, Egypt.
Ben-Amotz, A. and Avron, M. (1983).  Accumulation of metabolites by halotolerant algae and its industrial potential. Annual Review of Microbiology. 37: 95-119.
Borowitzka, M.A. and Borowitzka, L.J (1988). Dunaliella  in, Borowitzka, M.A. and Borowitzka, L.J. (eds). Microalgal Biotechnology. CambridgeUniv. Press, 27-58.
Chai-Ling Ho; Morphang, S. and Pang, T. (1995). Application of polymerase chain reaction (PCR) testing randomly amplified polymorphic DNA (RAPD) primers in the molecular identification of selected Sargassum species. Eur. I. Phycol. 30: 273-280.
Cowan, A.K.; Rose, P.D. and Horne, L.G. (1992). Dunaliella salina: A model system for studying the response of plant cell to response. J. Experimental. Botany. 43: 1535-1547.
Gobel, U. B.; Geiser, A. and Stanbridge, E.J. (1987). Oligonucleotides probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species. J.General Microbiology. 133: 1969-1974.
Gow, N.A.R. and Gadd, G.M. (1995). The growing fungus (eds. Chapman and Hall), London SEI 8HN, UK.
Hu, J. and Quiros, C.F. (1991). Identification of broccoli and cauliflower cultivars with RAPD markers. Plant cell Rep. 10: 505-511.
Ibrahim, S.A. and Abu Seada M.N. I. (1991). Phylogenetic relationship between various yeasts by means of isoelectric focusing of soluble protein in polysaccharide and agarose gels. Zagazig Agric. Res. 18(1): 69-82.
Ibrahim, S.A.; Laila, M.A. and Donhauser, S. (1990). Fingerprinting and alcohol dehydrogenase polymorphism in different yeasts. Egypt J. Genet. Cytol. 19: 131-142.
Jayarao, B. M.; Doré, J. E. ; Baumbach, G. A. ; Matthews, K. R. and Oliver, S. P. (1991). Differentiation of Streptococcus uberis from Streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analyses of 16S ribosomal DNA. Journal of Clinical Microbiology. 29: 2774-2778.
Livna, A.; Nelson, E.Y. and Sukenik, A. (1992). Immunological cross reactivity among photosynthetic proteins from various marine unicellular algal species. Botanica Marina. 35: 181-187.
Loeblich, L.A. (1982). Photosynthesis and pigments influenced by light intensity and salinity in the halophilic Dunaliella salina (Chlorophyta). J. Mar. Biol. Ass. U.K., 62: 493-508.
Markerd, C.L. and Moller, F. (1959). Multiple forms of enzymes tissue, onto genetic and specific patterns. Proceeding of NationalAcademy of Science of the USA. 45: 753-63.
Masjuk, N.P. (1973). Morfologija, Sistemetika, Ekologija, Geograficeskoe Rasprostranenie roda Dunaliella Teod. Naukova Dumka, Kiev. 244
Masjuk, N.P. and Radenko, M. I. (1973). New taxons from the genus Dunaliella Teod. III. Ukr. Bot. Zh. 30: 468.
Mohammad, Y.A. (1981). Biochemical taxonomy of Chlamydomonas and related volvocalean genera. Ph. D. thesis. University of California. Los Angeles. U.S.A.
Mohammad, Y.A. and Shaalan, S.H. (1985). Electroohoretic studies of isozymes in some Rhodophycean marine algae from Alexandria. Bull. Fac. Sci. Alex. Univ. 25: 10-22.
Neilan, B.A. (1995). Identification and phylogenetic analysis of taxigenic cyanobacteria by multiplex randomly amplified polymorphic DNA PCR. Applied and Environment Microbiology. pp.2286-2291.
Olmos, J.; Paniagua, J. and Contreras, R. (2000). Molecular identification of Dunaliella sp. Utilizing the 18S rDNA gene. Letters in Applied Microbiology. 30: 80-84.
Olsen, G.J.; Lane, D.J.; Giovannoni, S.J. and Pace, N.R. (1986). Microbial ecology and evolution: a ribosomal RNA approach. Annals of Review of Microbiology. 40:337-365.
Pontikis, C.A.; Loukas, M. and Kousounis, G.(1980). The use of biochemical markers to distingish olive cultivars. Journal of Horticulture Sciene. 55 (4): 333-343.
Ragan, M.A. and Chapman, D.J. (1978). A biochemical phylogeny of the  protists. Academic Press New York, San Francisco, London.
Rothe, G.M. (1994). Data evaluation population genetics and evolution in Rothe G.M. (ed.) Electrophoresis of enzymes. Springer Verlag BerlinHeidelberg. P 273.
Sambrook, K.T.; Frisch, E.F. and Maniatis, T. (1989). Molecular Cloning: a laboratory manual. Cold Spring Harbor, NY: ColdSpringHarbor Laboratory.
Scandalios, J.C. (1969). Genetic control of multiple molecular forms of enzymes in plants: a review. Biochem. Gen. 3:37-79.
Shaalan, S.H.; and Chapman, D.J. (1983 a). Chemotaxonomy of symbiotic Zoochlorella. I.Biochemical heterogeneity of isolates from different strains of Hydra viridis (Florida strain). Bulletin of the faculty of science, Alexandria.23: 41-59.
Shaalan, S.H.; and Chapman, D.J. (1983 b). Chemotaxonomy of symbiotic Zoochlo  rella. II. Effect of symbiotic process and culture conditions on the total soluble protein and isozyme profiles of some isolates from Hydra viridis (Florida strain). Bulletin of the faculty of science, Alexandria.
Shaalan, S.H.; and Chapman, D.J. (1983 c). Chemotaxonomy of symbiotic Zoochlorella. III.Biochemical heterogeneity of isolates from different strains of Hydra viridis and from different animal hosts. Bulletin of the faculty of science, Alexandria.23: 59.
Shaalan, S.H.; and Mohammad, Y.A. (1985). Chemotaxonomy of some marine algae belonging to family Ulvaceae from Alexandria sea shore. J. Fac. Mar. Sci. 4: 183-191.
Shaw, C.R. and Koen, A.L. (1964). Aspartate dehydrogenase activity of malate dehydrogenase. Biochim. Biophys. Acta. 92:397.
Shaw, C.R. and Prasad, R. (1970). Starch gel electrophoresis of enzymes-A compilation of recipes. Biochemical genetics. 4: 297-320.
Thomas, P. and Delcaripo, J.B. (1971).  Electrophoretic analysis of enzymes from 32 isolates of protosiphon. Phycologia. 9: 285-292.
Williams, J.G.K.; KubeliK, A.R. Rafalski, J.A. and Tingey, S.V. (1991). DNA polymorphism amplified by arbitrary are useful as genetic markers. Nuclic Acids Res. 18: 6531-6535.
Yamad, Y.; Aizawa, K.; Matsuoto, A.; Nakagawa, Y. and Bono, I. (1987). Electrophoretic comparison of enzymes in strains of fission yeasts genera Schizosacchromyces, Octosporomyces and Hasengawea. J. Appl. Microbiol. 33: 363-369.
Yang, X. and Quiros, C.F. (1993). Identification and classification of celery cultivars with RAPD markers. Theor. Appl. Genet. 86: 205-212.

Files

Share

How to cite

Statistics