A COMPARATIVE STUDY ON THE EFFECT OF EGYPTIAN AND LIBYAN CRUDE OIL EXTRACTS ON THE SYNTHESIS OF SOME METABOLITES OF DUNALIELLA BARDAWIL

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Alexandria University, Egypt

2 Biology Department, Faculty of Science, Omar El-Mokhtar University, Libya

Abstract

The effect of different concentrations of water soluble fractions of crude Egyptian and Libyan oil was tested on the synthesis of some metabolites of D. bardawil – a marine green unicellular alga- that used mainly for fish feeding. The results obtained cleared that toxic stress of crude oil extracts depends mainly on: type and source of oil, concentration of the extract and duration of culture period. Lower concentrations of both oil extracts stimulated synthesis of most measured metabolites while higher concentrations hampered their synthesis or even killed the organism. Libyan crude oil extract is more toxic than Egyptian one. Glycerol was the only metabolite that increased by increasing the concentration of the extract from 25% up to 75% of both types of oils. Total proteins, total carbohydrates and total amino acids content decreased quantitatively by increasing the concentration of extract and period of culture. At higher extract concentrations for both types of crude oils the number of gel bands for protein profile differed also according to the type of oil and concentration of extract. The zymogram obtained for the four studied isozymes cleared that toxicity effect of Libyan oil was more prominent than Egyptian oil.

Keywords

References

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