RADIONUCLIDE AND METAL BIOREMEDIATION FROM AQUATIC ENVIRONMENT BY ALGAE

Document Type : Original Article

Author

Department of Biological and Geological Sciences, Faculty of Education, Alexandria University.

Abstract

Three element were chosen for this investigation, radioactive carbon 14C  in form of 14C-urea and two element; chromium III and cobalt to study their biosorption and desorption mechanisms by two algal species;  Sargassum linifolium and  Dunaliella salina, in relation to their surface area. Active transport was the mechanism for 14C, Co and Cr uptake by the two algal species. Sargassum showed higher accumulation ratios than living algal cells; the reverse was true for Dunaliella. The antagonistic action between metal ions (radionuclide) binding to different algal cell surface was obtained by mixing 14C with Cr and 14C with Co in living algal cells of the two tested algae, while synergistic action was obtained by mixig Cr and Co in the living algal species. Non-interactive action was shown by mixng 14C with Cr or Co in dried algal material. 14C-uptake by living cells of Sargassum and Dunaliella ameliorated cell vitality; this effect was higher in Dunaliella than in Sargassum. Chromium showed adverse effect than cobalt, both Co and Cr altered the metabolic pathway of chlorophyll formation. Siderophore formation increased the adsorption power of algal cell-wall especially Sargassum alga. The biosorption effect of these radionuclides was due to surface characteristics. Desorption mechanism was fast from Sargassum surfaces, while it was slower in Dunaliella. Dried Sargassum can be used successfully for bioremediation of 14C, Co and Cr from the contaminated sea water (even, at low concentration) up to five elution times, while living Dunaliella could be used for the removal of these elements from lakes. To ameliorate the adsorptive power of dried Sargassum, its surface area must be increased and the media must be iron-free.

Akhtar, N.; Iqbal, J. and Iqbal, M. (2004). Removal and recovery of nickel (II) from aqueous solution by loofa sponge-immobilized biomass of Chlorella sorokiniana: characterization studies. J. Hazard. Mater., 108: 85-94
Amado filho, G. M.; Andrade, L. R.; Karez, C. S.; Farina, M. and Pfeiffer, W.C. (1999). Brown algae species as biomonitors of Zn and Cd at Sepetiba Bay, Rio de Janeiro, Brazil. Marine Environ. Res., 48 (3): 213-224.
Amorim, W. B.; Hayashi, M. A.; Pimentel, P. F. and Silva, M. G. (2003). A study of the process of desorption of hexavalat chromium. Braz. J. Chem. Eng. 20 (3): 213-227.
Bassham, J. A. and Calvin, M. (1957). The path of carbon in photosynthesis. prentice-Hall, Englewood Cliffs, New Jersey, USA.104pp
Bekheet, L. A. and Syrett, P. J. (1977). Urea-degrading enzymes in algae. Brit. Phycol. J., 12: 137-143.
Bekheet, I. A.; Barakat, S.Y.; Shaalan, S.H. and Shafik, M. A. (1984). Effect of nitrogen source on the uptake of urea by Scenedeumus obliquus. Com. Sci. Dev. Res., 7: 203-215.
Centinkaya Donmez, G.; Aksu, Z.; Ozturk, A. and Kutsal, T. (1999): A comparative study on heavy metal biosorption characteristics of some algae. Process Biochem., 34: 885-892.
Chaisukant, Y. (2003). Biosorption of cadmium (II) and copper (II) by pretreated biomass of marine alga Gracilaria fisheri. Environ. Technol., 24:1501-1508.
Gadd, G. M. (1990). Heavy metal accumulation by bacteria and other microorganisms. J. Announcement. 46:834-840.
Gazso, L. G. (2001). The key microbial processes in the removal of toxic metals and radionuclides from the environment. J. Chem. Tech. Biotechnol., 7(3-4): 178-185.
Halim, Y. (1987). Radiation pollution in the Mediterranean Sea. In: Symposium of Radiation Pollution. Alexandria Univ. Fac. Sci. Egypt. p20-25.
Jeffrey S. and Humphrey, G. (1975). New spectrophotometric equations for determining chlorophylls a, b, c and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanzen (BPP). 167: 191-194.
Jones, R. F. (1960). The accumulation of nitrosyl ruthenium by fine particles and marine organisms. Limnol. Oceanog., 5: 312-325.
Liu, Y., Yang, S. F., Tan, S. F., Lin, Y. M. and Tay, J. H. (2002). Aerobic granules: a novel zinc biosorbent. Letters in Applied Microbiology. 35 (6): 548-551.
Loeblich, L. A. (1982).Photosynthesis and pigments influenced by light intensity and salinity in the halophilic Dunaliella salina (Chlorophyta). J. Mar. Biol. Ass. UK. 62: 493-508.
Majidi, J.; Majidi, D. and Holcombe, J. A. (1990). Investigation of the metal algae binding site with Cd nuclear magnetic resonance. Environ. Sci. Technol., 249: 1309-1312.
Manley, S. (1984). Micronutrient uptake and translocation by Sacroeystis phyrifer. J. Phycol., 20:192-201.
Masjuk, N. P. (1972). On phylogeny and taxonomy of the genus Dunaliella salina Teod. Ukranskya Botanichnya Zhornal. 29:744.
Neilands, J. B. (1983): Siderophores. In: Advances in Inorganic Biochemistry. Vol.5. (L. Eichlorn and L. G. Marzilli, eds). Elsevier, North Holland, Amsterdam, pp. 137-166.
Pena-Castro, J.; Martinez-Jeronimo, F.; Esparza-Garcia, F. and Canizares-Villanueva, R. (2004). Heavy metals removal by the microalga Scenedesums incrassatulus in continuous cultures. Bioresource Technology. 94 (2): 219-222.
Phillips, D. (1990). Use of macroalgae and invertebrates as monitors of metal levels in estuaries and coastal waters. In: Furness, R. W. and Rainbow, P. S., Editors Heavy Metals in the Marine Environment, CRC Press, Boca Raton, FL, USA, pp. 81-99.
Price, L.W. (1983). The radioactivity counting handbook. Cambridge University
Rice, T.R. and Willis, V.A. (1959). Uptake, accumulation and loss of radioactive cesium-134 by marine planktonic algae. Limnol. Oceanog., 4: 277-290.
Saleh, A. M. (1987). Radiopharmaceuticals: uses and hazards. In: Symposium of Radiation Pollution. Alexandria Univ. Fac. Sci. Egypt. p32-38.
Shaban, N. Z. (1981). Biochemical studies of urea-degrading enzymes in green algae. M.Sc. Thesis, University of Alexandria, Egypt. 145pp.
Shafik, M.A. (1993). 14C-urea metabolism by some marine algae from Alexandria in combination with sewage treatment. Egypt J. Appl.  Sci., 8(10): 275-303.
Shafik, M. and Alaa, A. (1995). Nitrogen and phosphorus removal from industrial woolemater by the unicellular green alga Scenedesnus obliquus. Egypt. J. Appl. Sci., 10 (12): 307-319.
Spooner, G. M. (1949). Observations on the absorption of radioactive strontium and ruthenium by marine algae. J. Marine Bio. Ass., 28: 587-625.
Teresa, M.S.; Vasconcelos, D. and Fernanda, M. C. (2001). Seasonal variability in the kinetics of Cu, Pb, Cd and Hg accumulation by macroalgae. Marine Chemistry. 74 (1): 65-85.
Terry, P. A. and Stone, W. (2002). Biosorption of cadmium and copper contaminated water by Scenedesmus abundans. Chemosphere47: 249-255.
Tien, C. J. (2002): Biosorption of metal ions by freshwater algae with different surface characteristics. Process Biochem. 38: 605-613.
Valdmar, E. and Leite, S. (2000). Biosorption of Cal, Zn and Cu by Sargassum sp. waste biomass. Bioprocess Engineering. 22: 171-173.
Vilar, V. J.; Botelho, C. M. and Boaventura, R. A. (2007). Copper desorption from Glidium algal biomass. Water Research. 41(7): 1569-1579.
Volesky, B. and Kuyucak, N. (1988). Desorption of Cobalt-laden Algal. Biosorvent, Biotechnology and Bioengineering. 33:  815-822.    
Yang, J. and Volesky, B. (1999). Cadmium biosorbtion rate in prolonated Sargassum biomass. Environ. Sci. Technol., 33:751-757.