EFFECT OF TEMPERATURE ON THE ACTIVITY OF ACID PHOSPHATASES ISOLATED FROM CHLORELLA FUSCA, CLADOPHORA GLOMERATA AND PHORMIDIUM FAVEOLARUM AND THERMAL STABILITY OF THE ENZYMES

El-Shahed, A.; Ibrahim, H.; Abd El-Naeim, M.

doi:10.21608/egyjs.2008.114825

EFFECT OF TEMPERATURE ON THE ACTIVITY OF ACID PHOSPHATASES ISOLATED FROM CHLORELLA FUSCA, CLADOPHORA GLOMERATA AND PHORMIDIUM FAVEOLARUM AND THERMAL STABILITY OF THE ENZYMES

Document Type : Original Article

Authors

Botany Department, Faculty of Science, Minia University, Minia Egypt.

10.21608/egyjs.2008.114825

Abstract

Temperature effect has been investigated in this study on the phosphatases isolated from three algae representing different groups; a unicellular freshwater green alga Chlorella fusca, a filamentous green macroalga Cladophora glomerata and a filamentous freshwater blue green microalga, Phormidium faveolorum. The temperature dependence of the enzyme activity could be summarized as that 60 ºC was the optimum temperature for the enzyme activity in Cladophora glomerata as well as Chlorella fusca whereas Phormidium faveolorum had an optimum temperature of 70 ºC for both isolated enzyme fractions, the cell wall-bound and the secreted extracellular. The thermal behavior of the phosphatases isolated from the tested algae showed toleration to the thermal activation when p-nitrophenyl phosphate (pNPP) was utilized as a substrate which has been investigated to be itself is not sensitive to high temperatures. The two fractions of the enzyme isolated from the algae exhibited high stability at high temperatures even above 80 ºC

Keywords

References

Bhargava, R. and Sacher, R. C. (1987). Induction of acid phosphatase in cotton seedlings: Enzyme purification, subunit structure and kinetic properties, Phytochemistry, 26: 1293-1297.

Bold, H. and Wynne, M. (1978). Introduction to the algae. In: cultivation of algae in the laboratory. Bold H. and Wynee M. (Eds.), Printce-Hall,Inc., Englewood, Cliffs, New Jersy, pp: 571-578.

Feder, J. (1973). The phosphatase. In: Environmental phosphorus hand book, Griffith A. Beeton J.M. Spencer., D.T. Mitchell (eds), J. Wiley and Sons, PP: 475-508.

Ferreira, C.V.; Granjeiro, J. M.; Taga, E. M. and Aoyama, H. (1998). Soybean seed acid phosphatases: Unusual optimum temperature and thermal stability studies. Biochem. Biophys. Res. Commun., 242: 282-286.

Grimme, L. H. and Boardman, N. K. (1972). Photochemical activities of a partical fraction Pi obtained from green alga Chlorella fusca. Biochem. Biophys. Res. Commun., 49: 1619-1623.

Huber, A. L. and Kidby, D. K. (1984). An examination of the factors involved in determining phosphatase activities in estuarine water. 1: Analytical procedures. Hydrobiol., 111: 3-11.

Huber, A. L.; Huber, J. L. and McMichael, R.W. (1994). Control of plant enzyme activity by reversible phosphorylation. Int. Rev. Cytol., 149: 47-98.

Ibrahim, H.; Perti, H.; Pitterschatscher, K.; Fadl-Allah, E.; El- Shahed, A.; Bentrub, F. W. and Obermeyer, G. (2002). Release of an acid phosphatase during lily pollen tube growth involves components of secreted pathway. Protoplasma, 219: 176-183.

Ibrahim, H. (2005). Biochemical characterization of acid phosphatase from germinating pollen grains of Zea mays. J.Assuit Univ.Bot. 34(1): 371-382.

Jansson, M.; Olsson, H. and Pettersson, K. (1988). Phosphatases; origin; characteristics and function in lakes. Hydrobiol., 170: 157-175.

Kamenan, A. and Dipoh, J. (1982). Purification and physicochemical properties of an acid phosphatase from Dioscorea cayenensis cytoplasm. Plant Sci. Letters, 24: 173-182.

McComb, R.B.; Bowers, G. N. and Posen, S. (1979). Alkaline phosphatases. Plenum Press, NY, 986 PP.

Panara, F.; Pasqualini, S. and Antonilli, M. (1990). Multiple forms of barley root acid phosphatase : Purification and some characteristics of the major cytoplasmic isoenzymes. Biochim. Biophys. Acta, 1073: 73-80.

Park, H. and Van-Etten, R. (1986). Purification and characterization of homogeneous sun flower seeds acid phosphatase. Phytochem., 25: 351-357.

Pfeiffer, W. (1996). Auxin induce exocytosis of acid phosphatase in coleoptiles from Zea mays. Physiologia Plantarum, 98: 773-779.

Ullah, A. H. and Gibson, D.M. (1988). Purification and characterization of acid phosphatase from coleoptiles of germinating soybean seeds. Arch. Biochem. Biophys., 260: 514-520.