SEASONAL PRODUCTIVITY AND COSTS PRODUCTION OF DUNALIELLA BARDAWIL UNDER EGYPTIAN CONDITIONS

Document Type : Original Article

Author

Central Laboratory for Aquaculture Research, Abbassa, Ecology and Biology Department, Agricultural Research Center, Ministry of Agriculture, Cairo, Egypt

Abstract

Microalgae are the natural feeds of many aquaculture species and are the basis of the natural food. This work aimed at the investigation of the possibility cultivation green algae Dunaliella bardawil under outdoor conditions (Egyptian conditions) was studied, variation in chemical composition of algal cells due to season of cultivation was also investigated and costs production. Average cell mass productivity reached the maximum in summer followed by autumn and spring.  The algae were grown in batches using successively larger containers, the yield of the biomass, initial (1.5x106 cells/ml) and final density (12 x106 cells/ml)of  Dunalilla bardawil   were  obtained at four days culture 96 hours.  Methods of drying significantly affected amino acid composition.  Growth and chemical composition of Dunalilla bardawil cells are maximum crude protein content was around 52% for all seasons. Amino acid composition showed no significant difference between the batch cultures. Methionine was the limiting amino acid.  The calculated average and maximum productivity of algal cells and crude protein showed the highest value in summer culture followed by autumn and spring seasons. The chemical composition of the algal powder was similar for all batches in percent of dry weight, 51.8 % crude protein(C.P), 8.9 % ether extract (E.E), 6.5 % crude fiber (C.F), 9.2%  (ash),  23.6% N. free extract (NFE),and algal extracts containing antioxidant vitamins. The high cost of labor in the outdoor cultivation which represents approximately 49.26 % from the total operating costs, followed by the nutrients, which recorded about 26.10 % from the total costs, while the electricity had minimum cost recorded about 24.63 % from the total costs. The harvesting of ton lives algae and oven dried gave 950 g dry biomass. The 1.052 ton live algae produced 1kg dry biomass. The costs of Ingredient outdoor culture for producing  live  Dunalilla bardawil  and operating dry biomass  were about 15.30 Pound \ ton of live algae  and the costs of dry weight were LE 20.30 Pound, while operating costs were LE 21.37 Pound/kg dry biomass.

Keywords


A.O.A.C. Association of Official Analytical Chemists (1995). Official Methods of    Analysis Association of Official Analytical Chemists. Edit., KHL rich. Arlington Virginia.
Abd El-Hakim, N. F. and   Badawy, Tartiel M.E. (2008). Seasonal productivity and chemical composition of mass cultured Anabaena wisconsinense and Spirulina platensis  under Egyptian conditions. Egyptian Journal of Phycology9:211-233.
Allen, E. J. and Nelson, E.W. (1974). On the artificial culture of marine plankton organisms. J. Mar. Biol. Assoc., 8:421-474.
American Public Health Association (APHA) (1985).Standard methods for the examination of water and wastewater, edition American Public Health Association, Washington, D. C., USA.
Badawy, Tartiel M.E. (2005). Physiological studies on some green algae. Ph.D. thesis, Faculty of Agriculture, Cairo University. Egypt.
Badawy, Tartil M.E.  and Al-Kenawy, Diaa A. (2009). Utilization of blue green alga (Anabaena wisconsinense) as a feeding and biofertilization for Nile tilapia cultivation in rice fields. Egyptian Journal of Phycology, 10:367-381.
Badawy, Tartiel M.E.; Ibrahim, E. M. and Zeinhom, M. M. (2008). Partial    replacement of fish meal with dried microalga (Chlorella spp. and Scenedesmus spp.) in Nile tilapia (Oreochromis niloticus) diets. 8th  International Symposium on Tilapia in Aquaculture. (ISTA VIII), Cairo International Convention Center (CICC). Egypt, pp. 801-811.
Becker, E.W. (1994). Microalgae, Biotechnology and Microbiology.  Cambridge Univ. Press, pp. 9-39.
Behr, W. and C. J. Soeder. (2002). Economic aspects of the mass production of micro-algae. Proc. 2nd Egypt. Algae Symp., Cairo. pp. 72-85.
Bhumiratana, A. (1976). The production and utilization of Algae  as a Protein source in Thailand. Kasetsart University, Bangkok, 90 pp.
Bischoff, H . W . and Bold, H.C. (1963).  Phycological  studies. 4 some soil algae from Erchanted rock and related algal species. Univ. Texas, 8: 32-36.
Boyd, C. E. (l973).Summer algal communities and primary productivity in fish ponds. Hydrobiol., 41: 357-390.
Broun, W. (1980). Note on the survival of algal resting cells during long-term maintenance in darkness and minimum lake bottom temperature. Comparison of Anabaena, 5:677-680.
Dam, R.; Lee, S. K.; Fry, P.C. and Fox, H. M. (2002).Utilization of algae as a protein source for humans. J. Nutr., 86:376-382.
DeLorenzo, M. E.; Leatherbury, M.; Weiner, J. A. and Fulton, M. H. (2009).Physiological factors contributing to the species-specific sensitivity of four estuarine microalgal Exposed to the Herbicide Atrszine. Aquat. Ecosyst. Health Manage, 7:137-146.
De-Pauw, N. and G. Persoone. (2006). Microalgae for aquaculture. Algal   Biotechnology, Cambridge University, Press, New York. Pp.16-224.
De-Pauw, N.; Verboven, J. and Claus, C. (1998).Large-scale microalgae production for nursery rearing of marine bivalves. Aqua Cultural Engineering, 116/ 117:121-134.
Edmondson, W. T. (2006).Eutrophication in North America, eutrophication causes consequences. Washington, D.C., USA., 124-149.
El-Fouly, M. M.; Abdalla, F. E. and Saleh, A. M. (1984). Studies on out-door mass culture of green alga Chlorella vulgaris. Effect of nitrogen efficiency in growth medium. Al-Azhar Agric. Bull., 22:l-14.
El-Fouly, M. M.; Soeder, C. J.; Mohn, F. H. and Greoneweg, J. (1998).Open door mass production, chemical composition and biological evaluation of different algal species. Bull. Egypt, 2: 149-165.
Fan, L.; Vonshak, A. and Boussiba, S. (1994). Effect of temperature and irradiance on growth of Haematococcus pluvialis. J. Phycol., 30:829-833.
FAO (1999).FAO training series 21/1. Simple methods for aquaculture, management for fresh water fish culture and water practices, Rome, Italy.
Fee, E.J. (2006). A relation between lake morphometry and primary productivity and its use in interbreeding whole. Lake eutrophication experiments. Limnol. Oceanger., 24: 401-416.
Felts, P.A. and Heath, A.G. (1984).Interactions of temperature and sublethal environmental copper exposure on the energy metabolism. J. Fish Biol., 25: 445-453.
Fogg, G.E. (1984). Algal cultures and phytoplankton ecology. University of Wisconsim Press, pp. 37-51.
Fulks, W. and Main, K. L. (1996). The design and operation of commercial scale live feeds production system. Fisheries Research Technical. 1:16-20.
Hemerick, G ; Hoshow, R. W. and Rosowski, J. R. (1973): Methods for algae, Handbok of phycological methods and growth measurements. Cambridge university press, London, pp:53-68.
Munoz, R.; Kollner, C.; Guieysse, B. and Mattiasson, B. (2004).Photosynthetically oxygenated salicylate biodegradation in a continuous stirred tank photobioreactor. Center for Chemistry and Chemical Engineering, Lund University, 6:797-803.
NRC (Nutrient Requirements of Fishes) (1993).National Research Council, Nutrient Requirements of Domestic Animals, 128 pages.
Persoone, G.; Morales, J. and Claus, C. (1988). Mass culture of algae bottleneck in the nursery culturing of molluscs. Algae biomass.Elsevier/North-Holland Biomedical Press, New York., USA.
S. A. S. Statistical Analysis Systems. (2000). SAS program Ver. 6. 12,SAS institute incorporation. Cary. NC 27513 USA.
Shelef, G.; Moraine, R. and Oron, G. (2005). Economic aspects of microalgae production " microalgae for food and feed " Limnol. 201-204.
Soeder, C. J. (2003). The technical production of microalgae and prospects in marine aquaculture. Plenum Press. New York, 39-55.
Spectorova, A.; Montesinos, J. L.; Cusido J. A. and Godia, F.  (1997). Recovery and treatment of Spirulina  platensis cells cultured in a  continuous photobioreactor to be used as food. Process Biochem., 37: 535-547.
Taha, E. M. and Allam, A. M. (1959). Physiological and biochemical studies on Egyptian fresh water algae. V. Growth and cell protein influenced by culture conditions. Arch. Microbiol., 34:393-400.
US EPA; United States Environmental Protection Agency (2007). National Recommended Water Quality Criteria Correction Office of Water, EPA 822-z-99-001, 25 pp.
Wang, S.M.; Wang, Q.L.; Li, S.H. and Zhang, J. R. (2007).  A study of treatment of spring wheat with growth promoting substances from nitrogen-fixing blue-green algae. Academia Sinica, Wuhan, Hubei, China,1:45-52.
Yan, G.; Yu-Jing, Y.; Wang, X. and Wang, Y.X. (2000).  The effects of pH and   temperature on orthophosphate removal by immobilized Chlorella vulgaris. Biotechnology Letters, 18(8):893-896.
Yang, J. X. and Huang, X.F. (2007).  Effects of environmental factors on the hatching rate of eggs (Rotatoria: Monogononta). Acta Hydrobiologica Sinica, 4: 331-339.