COUNTERACTING INHIBITORY EFFECT OF SALINITY STRESS ON COWPEA GERMINATION USING CYANOBACTERIAL EXTRACTS

Zeid, Ibrahim Mohamed; Ghazi, Safia Mohamed; El Semary, Nermin Adel; Abd El-Sattar, Amira Mohamed

doi:10.21608/egyjs.2014.115236

COUNTERACTING INHIBITORY EFFECT OF SALINITY STRESS ON COWPEA GERMINATION USING CYANOBACTERIAL EXTRACTS

Document Type : Original Article

Authors

Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt.

10.21608/egyjs.2014.115236

Abstract

The response of cowpea seeds grown under salinity-induced stress conditions to different cyanobacterial extracts of two strains (Aphanizomenon flos-aquae and Phormidium sp.) was studied during seed germination. The results indicated that aqueous extract was more stimulating to germination than organic extract where higher levels of germination percentage, enzymatic activity, nucleic acid, protein and total soluble sugars contents were obtained. In addition, the indicators of stress such as proline content, lipid-peroxidation and relative permeability of the root membranes were lower than the organic extract. Application of aqueous cyanobacterial extracts of Aphanizomenon flos-aquae and Phormidium sp. stimulated seed germination and the metabolic activities of salt-stressed and unstressed seeds, while the lipid-peroxidation and relative permeability of the root membranes were reduced. Therefore, aqueous algal extracts alleviated the harmful effect of salinity stress on seed germination. This can be used on a vast scale as an inexpensive and eco-friendly farming policy to counteract the hazardous effect of salinity on plants especially during the critical period of seed germination where salinity can inhibit it either partially or completely.

Keywords

References

Abd El Baky, H. H.; Hussein, M. M. and El-Baroty, G. S. (2014). Induces of antioxidant compounds and salt tolerance in wheat plant irrigated with seawater as response to application of microalgae spray. American Journal of Agricultural and Biological Sciences 9 (2): 127-137.

Abd El-Baky, H. H.; Hussein, M. M. and El-Baroty, G. S. (2008). Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant irrigated with sea water. African Journal of Biochemistry Research 2 (7):151 –164.

Adam M. S. (1999). The promotive effect of the cyanobacterium Nostoc muscorum on growth of some crop plants. Acta Microbiology Polo 48:163–71.

Azaizeh, H. and Steudle, E. (1991). Effects of salinity on water transport of excised maize (Z. mays L.) roots. Plant Physiol97:1136–45.

Azaizeh, H.; Gunse, B. and Steudle, E. (1992). Effects of NaCl and CaCl2 on water transport across root cells of maize (Zea mays L.) seedlings. Plant Physiol99:886–894.

Baset, M. M. A. and Shamsuddin, Z. H. (2010). Rhizobium as a crop enhancer and biofertilizer for increased cereal production. African Journal of Biotechnology 9(37): 6001-6009.

Bates, L. S.; Waldren, R. P. and Teare, I. D. (1973). Rapid determination of free proline for water stress studies Plant and Soil. 39: 205-207.

Bergmeyer, H. U. (ed) (1974). Methods of Enzymatic Analysis. Verlag Chemic, Weinheim, Academic Press, New York London

Bernheim, F.; Bernheim, M. L. C. and Wilbur, K. M. (1948). The reaction between thiobarbituric acid and the oxidation product of certain lipids.J BiolChem 174: 254-264.

Chen, X. L. and Zhao, K. F. (1996). Effect of NaCl stress on germination of maize seeds and the alleviation of exogenous Ca²⁺. Acta Agriculturae Boreali Sinica 11(4): 89-92.

Clyde, W.; Liu, X.; Lesch, S. M. and Suarez, D. L. (2006). Growth response of major USA cowpea cultivars II Effect of salinity on leaf gas exchange. Plant Science 170:1095–1101.

Dische, Z. and Scwartz, Z. (1973). Thin Layer Chromatography. Micochin Acta, 2, 13 Cited by: E Stahal (ed) 2^nd Spriger Verlage Berlin

Dkhil, B. B. and Denden, M. (2010). Salt stress induced changes in germination, sugars, starch and enzyme of carbohydrate metabolism in Abelmoschus esculentus L. (Moench) seeds African Journal of Agricultural Research 5(12):1412-1418.

El-Gamal, D.; Ghanem, A. E. N.; El-Ayouty, E. Y. and El-Belely, E. F. (2008). Differential responses of Anabaena Variabilis and NostocLinckia to salt stress and their role in improvement the growth conditions of some salt stressed plant seedlings.International Conference for Enhancing Scientific Research: New HorizonsTanta Conference1-10

Feuillade, J. (1994). The cyanobacterium (blue green alga). Oscillatoria rubescence DC Archives of Hydrobiology 42: 77-93.

Gadwal, R. and Naik, G. R. (2014). A comparative study on the effect of salt stress on seed germination and early seedling growth of two Hibiscus species. Journal of Agriculture and Veterinary Science.7:(3) 90-96.

Hoque, M. A.; Okuma, E.; Nakamara, Y.; Shimoishi, Y. and Murata, Y. (2008). Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaClinduced damage in cultured tobacco cells. J Plant Physiol 165:813–824.

Hu, Y. C.; Fricke, W. and Schmidhalter, U. (2005). Salinity the growth of non-halophytic grass leaves: the role of mineral nutrient distribution. Chair of Plant Nutrition Functional Plant Biology 32(11): 973-985.

Kay, R. A. and Barton, L. L. (1991). Microalgae as food and supplement. Critical Reviews in Food Science and Nutrition 30 (6): 555–573.

Khan, M. H. and Panda, S. K. (2008). Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress. Acta Physiol Plant 30:91-89.

Kishor, P. B. K.; Sangam, S.; Amrutha, R. N.; Laxmi, P. S.; Naidu, K. R. and Rao, K. S. (2005). Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci (88): 424–438.

Kumar, G. and Sahoo, D. (2011). Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold. J Appl Phycol23: 251–255.

Marmur, J. (1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. Bot J 208-18.

Munns, R. (2002). Comparative physiology of salt and water stress.Plant Cell and Environment25: 239–250.

Oktem, H. A.; Eyidogan, F.; Selcuk, F.; Silva, J. A. T. D. and Yucel, M. (2006). Osmotic stress tolerance in plants: transgenic strategies. Floriculture Ornamental and Plant Biotechnology 194-208.

Palaniappan, P.; Malliga, P.; Manian, S.; Madhaiyan, S. S. M. and Sa, T. (2010). Plant growth promontory effect on Cow pea (Vigna unguiculata L) using coir pith aqueous extract formulation of cyanobacterium Phormidium. American-Eurasian J Agric and EnvironSci 89(2): 178-184.

Sharma, S. S. and Dietz, K. J. (2006). The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot. 57:711–726.

Shehata, M. M. and El-Khawas, S. A. (2003). Effect of two biofertilizers on growth parameters, yield characters, nitrogenous components, nucleic acids content, minerals, oil content, protein profiles and DNA banding pattern of sunflower ( Helianthus annus L cv Vedock) yield. Pakistan Journal of Biological Sciences 6(14):1257:1268.

Sheoran, I. S. (1980). Changs in amylase during germination and early seedling growth of mung bean (Vigna radiate l). Eilczek under different salts Indian J Plant Phsiol 23: 169-173.

Siam, H. (2008). Increasing the efficiency of utilization of nitrogenous fertilizers through addition of Banana compost on growth, yield and nutrients of cowpea plants. Egypt J of Appl Sci 23(7): 54-6.

Sivakumar, P., Sharmila, P. and Saradhi, P. P. (2000). Proline alleviates salt-stress induced enhancement in Rubisco oxygenase activity. Biochem Biophys Res Commun 279:512–515.

Snedecor, G. W. and Cochran, W. G. (1980). Statistical Methods. 6^th Ed. Iowa State University Press Ames

Thakur, M. and Sharma, A. D. (2005). Salt stress and phytohormone (ABA) induced changes in germination, sugars and enzymes of carbohydrate metabolism in Sorghum bicolor (L) Moench seeds. JAgric Soc Sci 1: 89-93.

Umbriet, W. W.; Burris, R. H.; Stauffer, J. F.; Cohen, P. P.; Johanse, W. J.; Lee, P. G. A.; Potter, V. R. and Schneider, W. C. (1959). Monometric technique, a manual descripton method, applicable to study of desiring metabolism. P 239 Burgess Publishing Company (cf Razak AA 1979)

Venkataraman, G. S. (1981). Blue-green algae for rice production-a manual for its promotion. FAO Soils Bull, 46: 1-52.

Zayed, M. A. and Zeid, I. M. (1998). Effect of water and salt stresses on growth, chlorophyll, mineral ions and organic solutes contents and enzymes activity in mungbean seedlings. Biol Plant 40: 351-356.

Zeid, I. M. (2004) Responses of bean (Phaseolus vulgaris) to exogenous putrescine treatment under salinity stress. Pakistan J Biol Sci 7(2): 219-225.

Zeid, I. M. (2009). Effect of arginine and urea on polyamines content and growth of bean under salinity stress. Acta Physiol Plant, 31: 65-70.

Zeid, I. M. (2011). Allevation of seawater stress during germination and early growth of barley. International Journal of Agriculture: Research and Review, 1 (2): 59-67.

Zwiazek, J. J. and Blake, T. J. (1991). Early detection of membrane injury in black spruce (Picea mariana). can J for Res 21: 401-404.