Effect of Fluazifop-P-butyl Herbicide on Carbohydrate, Protein, and Fatty acid compositions of Auxenochlorella pyrenoidosa and Raphidocelis subcapitata

Aboelgalagel, Elsayed Mohamed; El-Abasy, Manal Mohamed; Abd El Kareem, Mohamed Saad; Abdel-Halim, Khaled Yassin; Mohy El-Din, Soad Mohamed

doi:10.21608/egyjs.2025.398709.1049

Effect of Fluazifop-P-butyl Herbicide on Carbohydrate, Protein, and Fatty acid compositions of Auxenochlorella pyrenoidosa and Raphidocelis subcapitata

Document Type : Original Article

Authors

¹ Botany and Microbiology Department, Faculty of Science, Alexandria University

² Mammalian & Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, (CAPL), Agricultural Research Center (ARC), Giza, Egypt

³ Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt

10.21608/egyjs.2025.398709.1049

Abstract

Herbicides have been a major issue in terms of environmental and human safety; thus, a greater interpretation of the influence of this herbicide on the aquatic environment is necessary. In this study Auxenochlorella pyrenoidosa is treated with two sublethal concentrations from each form of fluazifop-P-butyl, 0.237 and 1.09 mg/L from traditional fluazifop-P-butyl (TFL) and 0.357 & 1.43 mg/L from nano fluazifop-P-butyl (NFL). Also, Raphidocelis subcapitata is treated with 0.029 & 0.117 mg/L from TFL and 0.119 & 0.476 mg/L from NFL. Total carbohydrate, protein, lipid contents and fatty acid compositions in both microalgal cells were evaluated after exposure time (96 h). Results showed that treated cells of R. subcapitata with 0.117 mg/L of TFL increased carbohydrate content by, 78.87% when compared to the control. Treated cells of A. pyrenoidosa with 1.43 mg/L of NFL showed the highest decrease in total protein content (76.04%) as compared to the control. Total lipids increased by 136.22% in cells of A. pyrenoidosa treated with 1.09 mg/L of TFL compared to the control. Treated cells of A. pyrenoidosa with 0.273 mg/L of TFL showed an increase of total saturated and total unsaturated fatty acids by 118.71% and 186.85%, respectively, compared with the control. Overall, this study shows that TFL and NFL herbicide impacted on some biochemical components of the two studied algae, thereby altering the nutritional quality of this resource for primary consumers. Also, this study may enhance the possibility of using fatty acids as biomarkers of herbicide exposure assessment in freshwater ecosystems.

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